Help on Prokayrotic Protein Expression
Ricardo.Silva at CIGB.EDU.CU
Wed Oct 14 16:09:27 EST 1998
If the codon usage of your 40 kDa protein is too different from the
usage of E. coli, problems often appear. Arg-rich and very
hydrophobic tracts also hamper expression in bacteria. Sensitivity to
degration might also decrease the amount of the product expected.
Hope this is helpful,
DIV. VACUNAS, CIGB,
> I cloned the cDNA ...and have tried to produce this protein...
However, I have been getting very little expression...
This protein is reported to be non-toxic...
> Aki Hoji
(akhst7+ at pitt.edu)
> Dept. Infectious Diseases & Microbiology
> University of Pittsburgh
More information about the Methods