genomic DNA isolation from whole cells without precipitation
woodlab at SUNGCG.USOUTHAL.EDU
Wed Oct 14 15:19:26 EST 1998
I am looking for a protocol or resin that will allow me to
isolate genomic DNA from whole cells without having to precipitate.
Here's the deal, I am doing preps from a 96 well plate. Our equipment
won't spin these plates more than 3000xg, not quite enough for EtOH prec.
As it is, I have to transfer the 96 samples to eppnd. tubes, lyse,
phenol/chloroform extract, then precipitate. The prep doesn't have to be
exceptionally clean, I'm just going to PCR. But, the amount of DNA is
small compared to all the junk, so I have to clean it up somehow.
Any suggestions will be appreciated. You can e-mail me directly at
woodlab at sungcg.usouthal.edu
Department of Microbiology
University of South Alabama
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