Southern blot with tailing oligo probes

[tim]

Hi everybody, 

I'm looking for a working protocole (buffer, wash...) for Southern blot
(on digested genomic DNA) with hybridization with an oligonucleotide
probe (labeled by tailing). I have some problems. At this time, I'm
using the same technique as  hybridization, but it didn't work.

Many thanks for your help

Dr Christophe FERRAND
ETS de Franche ComtŽ
1 Bd A. Fleming
25020 Besancon cedex
Phone : 33 3 81615615
Fax : 33 3 81615617
E-mail : tim at besancon.net