Southern blot with tailing oligo probes

tim tim at
Wed Oct 14 03:13:29 EST 1998

Hi everybody, 

I'm looking for a working protocole (buffer, wash...) for Southern blot
(on digested genomic DNA) with hybridization with an oligonucleotide
probe (labeled by tailing). I have some problems. At this time, I'm
using the same technique as  hybridization, but it didn't work.

Many thanks for your help

Dr Christophe FERRAND
ETS de Franche ComtŽ
1 Bd A. Fleming
25020 Besancon cedex
Phone : 33 3 81615615
Fax : 33 3 81615617
E-mail : tim at

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