Hello all, >E.coli W3110, in particular it is supE and/or supF? I found this in an old workbook, but cannot remember the source from where I copied: W3110 genotype: thyA36, deoc2, IN(rrnD-rrnE)1, rph, pyrE, Lambda-F-. Hope it is helpful, Best wishes, ORLANDO, DIV. VACUNAS, CIGB, HABANA, CUBA.