Non-radioactive sequencing method ??
delete at leeds.ac.uk (Hedley Carr
delete at leeds.ac.uk (Hedley Carr
Thu Oct 15 08:15:58 EST 1998
Hi all,
I'm looking for a non-radioactive DNA sequencing method that fulfils
the following criteria:
-Rapid to perform
-Cheap
-Base reading only need be 100-150 bp per lane.
OK, I'm dreaming, but I'm going to be sequencing rather a lot of short
sequences so massive reads aren't necessary. I would also prefer
non-radioactive methods because:
a) I value my gonads
b) Radioactive materials are a pain to handle and are useless after a
month of storage.
I've checked out a Cycle-sequencing method with silver-staining but
that didn't work particuarly well last time (poor signal to noise
ratio).
Any ideas?
Thanks,
Hedley Carr.
P.S. To reply via e-mail, remove the 'XXX' from my address.
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