ISH or FISH?

Frauke Naumann fnaumann at scan.genetik.uni-koeln.de
Thu Oct 15 04:30:13 EST 1998


Warren Raye schrieb in Nachricht
<36248458.ADDBA2DA at numbat.murdoch.edu.au>...
>Can anyone give me
>a defiinnitive answer on which  is better ISH or FISH?
>Please reply be email if possible.

Hi Warren and netters,
"nothing" in life is "ever" definitive....
Anyway, FISH can be much more sensitive, because you can amplify your signal
via a "sandwich" reaction: 1. Anti-DIG-FITC out of sheep (for example) 2.
rabbit- anti-sheep-AB 3. anti-rabbit-AB labeled with FITC. Severel
anti-sheep bind anti-DIG .....
:-)
Frauke





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