DDRT-PCR and silver staining

KwangChul Oh ohk at ucs.orst.edu
Thu Oct 15 04:15:30 EST 1998


I am planing to use silver staining kit to detect DDRT-PCR product on
TBE-urea gel. The PCR-product will be reamplified to make probe. I am
concerned whether fixative containing acid such as acetic acid, TCA and
sulphosalicylic acid may modify or destroy the bases. Do I have to use
isotope instead of silver staining?

Thank you for help


KwangChul Oh

ohk at bcc.orst.edu

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