cross-linking

Alex Hutagalung ah692010 at bcm.tmc.edu
Thu Oct 15 23:24:38 EST 1998


Hi!
When doing cross-linking experiments, what are good controls to do?  I
have the reagent DSP which I understand can be reduced with DTT to
separate cross-linked proteins in later steps for analysis.
Additionally, does DSP need some sort of inducing reagent to
cross-link?  This is for my qualifying exam and it involves the reaction
of a chaperone with a protein for those who are wondering.  Thanks.




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