?Peptide sample preparation for mass spec?

Jongrak Kittiworakarn g3937506 at STUDENT1.MAHIDOL.AC.TH
Mon Oct 19 06:21:34 EST 1998


Dear all:

I have a peptide <6 kDa which is obtained from trypsin digestion of the
native protein.
The peptide seemed to be bind to the other trypsis resistant domain of
the native protein.
I would like to analyse this peptide by electrospray mass spec. How
should I prepared this sample for the MS.

Is it possible to do as follows:
First, separate the peptide from other peptides/proteins by
electrophoresis. Then, transfer it to nitrocellulose membrane, and cut
the band of interest. Dissolve the membrane in acetone and separate the
peptide by precipitation.

Thanks for any suggestion.

Jongrak

--
-----------------------------------Mr. Jongrak Kittiworakarn
                                   g3937506 at student.mahidol.ac.th

     Inst. of Science and Technology for Research and Development
     Mahidol University (Salaya Campus)
     Salaya, Nokornprathom, THAILAND.
     Tel. 001-66-2-441-9003-7 ext.1277,1278,1279
     Fax. 001-66-2-441-9906, 001-66-2-441-1013
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