DNA and autoclaving
Richard P. Grant
see_sig at cmtech.co.delete.uk
Wed Oct 21 04:47:00 EST 1998
In article <362DA56E.17FF at sheffield.ac.uk>, R U Gianfrancesco
<BOP95RUG at sheffield.ac.uk> wrote:
: I'm using DNA as a food source to fungi. I have autoclaved my salmon
: sperm DNA but do not know the effect of autoclaving. Does anyone know?
: Such as single/double strand, bonding, strand size? thanks.
Let's put it this way - if you autoclave genomic DNA you can still do PCR
from it. It's tough stuff. There is no reason - aside from mechanical
stress - that you should get strand breakage. I guess that a/c will
separate the strands, but they will renature as it cools.
Richard P. Grant MA DPhil | rgrant at cmtech.co.uk
Senior R&D Scientist | work: www.cmtech.co.uk
Cambridge Molecular | home: www.avnet.co.uk/adastra
-- 'Practising biochemistry without a licence' - E. Chargaff --
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