Lambda DNA migration in agarose gels?

Jens Mollerup jmollerup at aki.ku.dk
Thu Oct 22 06:50:17 EST 1998


Hi netters,
We have purified lambda ZAP ll DNA with a size of 40kb. Surpricingly,
when runned on an 0.6% agarose gel this fragment goes as a 20kb band?
After one cut with a restriction enzyme two bands at approx 20kb
appears. Does anyone have an explanation for these observations?
Thanks in advance

Jens Mollerup




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