His tag protein molecular weight

Ke-xue Huang khuang at BIOC.RICE.EDU
Thu Oct 22 12:15:47 EST 1998


Hi, Neuters,

We are trying to detect the molecular weight of Histag expressed protein
using HPLC gel filtration column, but the proteins come out much smaller
than that of its immigration on SDS-PAGE. Any suggestion?

Thanks!

Kexue




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