In situ bacterial DNA quantitation?

Richard P. Grant see_sig at
Thu Oct 22 10:19:21 EST 1998

In article <362f4062.0 at>, toukie at (Dr. S.
Shapiro) wrote:

:      I am curious if anyone is aware of a procedure for quantifying the
: DNA (or total nucleic acid) in individual bacterial cells that does not
: make use of radiosiotopes or flow cytometers.  For example, can it be done
: microscopically (electron microscopically) using particular fluorescent
: dyes?  Any other suggestions?


You might try crystal violet.  Make up a 10% solution in methanol and use
at about 1/1000 in water.  Rinse off in water.  No refs to hand but search
on PubMed - - for 'crystal violet'.



Richard P. Grant MA DPhil     |             rgrant at
Senior R&D Scientist          |             work:
Cambridge Molecular           |      home:           
 -- 'Practising biochemistry without a licence' - E. Chargaff --

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