In situ bacterial DNA quantitation?
Richard P. Grant
see_sig at cmtech.co.delete.uk
Thu Oct 22 10:19:21 EST 1998
In article <362f4062.0 at rzunews1.unizh.ch>, toukie at zui.unizh.ch (Dr. S.
: I am curious if anyone is aware of a procedure for quantifying the
: DNA (or total nucleic acid) in individual bacterial cells that does not
: make use of radiosiotopes or flow cytometers. For example, can it be done
: microscopically (electron microscopically) using particular fluorescent
: dyes? Any other suggestions?
You might try crystal violet. Make up a 10% solution in methanol and use
at about 1/1000 in water. Rinse off in water. No refs to hand but search
on PubMed - http://www.ncbi.nlm.nih.gov/PubMed/ - for 'crystal violet'.
Richard P. Grant MA DPhil | rgrant at cmtech.co.uk
Senior R&D Scientist | work: www.cmtech.co.uk
Cambridge Molecular | home: www.avnet.co.uk/adastra
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