In situ bacterial DNA quantitation?

Richard P. Grant see_sig at cmtech.co.delete.uk
Thu Oct 22 10:19:21 EST 1998


In article <362f4062.0 at rzunews1.unizh.ch>, toukie at zui.unizh.ch (Dr. S.
Shapiro) wrote:

:      I am curious if anyone is aware of a procedure for quantifying the
: DNA (or total nucleic acid) in individual bacterial cells that does not
: make use of radiosiotopes or flow cytometers.  For example, can it be done
: microscopically (electron microscopically) using particular fluorescent
: dyes?  Any other suggestions?


Hi,

You might try crystal violet.  Make up a 10% solution in methanol and use
at about 1/1000 in water.  Rinse off in water.  No refs to hand but search
on PubMed - http://www.ncbi.nlm.nih.gov/PubMed/ - for 'crystal violet'.

rgds,

Richard

-- 
Richard P. Grant MA DPhil     |             rgrant at cmtech.co.uk
Senior R&D Scientist          |             work: www.cmtech.co.uk
Cambridge Molecular           |      home: www.avnet.co.uk/adastra           
 -- 'Practising biochemistry without a licence' - E. Chargaff --



More information about the Methods mailing list