pBR322 with MCS?

Scott Henderson scotth at physci.ucla.edu
Thu Oct 22 18:15:49 EST 1998


Does anyone know of a plasmid with a pBR322 ori (or other semi-low copy
ori) *AND* a nice MCS for the purpose of subcloning and growing up larger
DNA inserts (16 kb)?  At least several groups report that large constructs
in a relatively low copy number plasmid are much more stable in E. coli
than when in free, hip, and loose plasmids.

Thanks in advance.

Scott Henderson, PhD
Physiology, UCLA, USA
scotth at physci.ucla.edu



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