PCR to Agarose Gel Shortcuts

weazel at blarg.net weazel at blarg.net
Fri Oct 23 16:32:14 EST 1998


You really don't need all that spinning for adding loading dye. Just add the
dye beneath the oil layer and it will mix in with the reaction on it's own.
Don't worry about contamination unless you're planning on re-amplifying it.

Another shortcut for loading PCR products onto agarose gels is to load the
gel before submerging it. Much, MUCH easier this way if there's oil on top.
Just be careful to slowly submerge the loaded gel to keep the samples from
washing out.

beth

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