His tag protein molecular weight

Tobias Straub tobias.straub at mail.uni-wuerzburg.de
Fri Oct 23 15:57:52 EST 1998

In article <36307A9B.AC151E38 at pitt.edu> , Rich Dudley <rdudley+ at pitt.edu> 

>Anomolous migration of his-tagged proteins on SDS-PAGE is well known.
>Take a look at the archives of this newsgroup for some more useful info.

and one should always take into accout that gel filtration columns separate
native (e.g. globular) proteins (if the proteins are not denatured before
loading). SDS-PAGE separates denatured proteins.

Tobias Straub
Medizinische Poliklinik, University of Wuerzburg
Klinikstrasse 6-8, 97072 Wuerzburg, Germany
Phone: +49-931-2017085      Fax: +49-931-2017120
Tobias.Straub at mail.uni-wuerzburg.de

More information about the Methods mailing list