His tag protein molecular weight

Tobias Straub tobias.straub at mail.uni-wuerzburg.de
Fri Oct 23 15:57:52 EST 1998


In article <36307A9B.AC151E38 at pitt.edu> , Rich Dudley <rdudley+ at pitt.edu> 
wrote:

>Anomolous migration of his-tagged proteins on SDS-PAGE is well known.
>Take a look at the archives of this newsgroup for some more useful info.
>

and one should always take into accout that gel filtration columns separate
native (e.g. globular) proteins (if the proteins are not denatured before
loading). SDS-PAGE separates denatured proteins.

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Tobias Straub
Medizinische Poliklinik, University of Wuerzburg
Klinikstrasse 6-8, 97072 Wuerzburg, Germany
Phone: +49-931-2017085      Fax: +49-931-2017120
Tobias.Straub at mail.uni-wuerzburg.de
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