help, Ab Staining.
yjx at PUBLIC.EAST.CN.NET
Sun Oct 25 20:05:10 EST 1998
Can some one help me? my friend encounter a confusing problem while doing the label and
stain with the cells on slide. he had succeed with those AB on Cos
Cells for Transient expression assay, but when he turn to the MDBK
cells that may have the intergrated gene coding the target protein
and just have the posibility to express it. he failed to see
anything in the fluoresence microscope. it seems that the Antibody
does not bind to the cell at all. Does any noe know why? is there
any difference betweewn the Cos cells and the MDBK cells?it is a
little confusing that the AB works well at the transivnt assay, but
does have no effectin to MDBK cells. And what may affect the result
during the work?
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