DNA from Soil

[Gary and Andrea DosSantos]

Hello,

If the PCR primers were not designed to be specific to a single organism,
it could be that rDNA from more than one organism was amplified. The
resulting amplicons would be of the same size, but with differing
sequences.

MOUNIR IZALLALEN wrote:

> HI,
>
> My colleague succesfully extracted DNA from a soi, amplify a 16S region
> and
> purify the
> PCR product. But when he digest with EcorI, he  gets a smear, while he
> was
> expecting 3 to 4 bands.
>
> Do you have any suggestion regrding this.
>
> Mounir Izallalen
> RSVS
> universite laval