Ask for Klenow

ma ddsyr at PUBLIC.EAST.CN.NET
Tue Oct 27 08:29:44 EST 1998


Dear Netters:
Anyone have such experiences: I want to delete a BamHI site in a
promoter, so I plan to digest with BamHI then treat with Klenow then
ligase. But I wonder if this serial of treatment will affect the
promoter activity? Thanks for your information.
Dr Q. H. Ma
Institute of Botany, Academia Sinica
Beijing 100093, China




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