Ask for Klenow

ma ddsyr at PUBLIC.EAST.CN.NET
Tue Oct 27 08:29:44 EST 1998

Dear Netters:
Anyone have such experiences: I want to delete a BamHI site in a
promoter, so I plan to digest with BamHI then treat with Klenow then
ligase. But I wonder if this serial of treatment will affect the
promoter activity? Thanks for your information.
Dr Q. H. Ma
Institute of Botany, Academia Sinica
Beijing 100093, China

More information about the Methods mailing list