neomycin-G418 confusion

Wolfgang Schechinger Wolfgang.Schechinger at med.uni-tuebingen.de
Wed Oct 28 11:56:56 EST 1998


Hello Martin, 

The story about Neomycin and G418 is this: 

1. The NeoR gene is coding for neomycin phosphotransferase
2. Neomycin binds to ribosomes in certain Prokaryotes
3. G418 binds ... Eukaryotes
4. Neomycin phostransferase phosphorylates both G418 and Neomycin and 
renders them inactive.
5. G418 is only effectice in Eukaryotes
6. Neomycin ... Prokaryotes. 

This all is written down nowhere. It seems to be 
common sense <GG>.  

7. Maybe those guys who are writing the catalogues are reading this 
message here and will include these hints in every catalogue.

Wolfgang 
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usual disclaimers apply * This message is RNAse free - please don't touch!
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Wolfgang Schechinger         
University of Tuebingen, Germany
email: wgschech at med.uni-tuebingen.de * wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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