Another PCR Loading buffer question
Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Wed Oct 28 05:09:58 EST 1998
In article <36365A7A.919D750D at sph.unc.edu>, John Scott Meschke
<jmeschke at sph.unc.edu> writes
>Second, it is clear from responses to my first posting that bromophenol
>blue is not compatible with PCR, but is it photoreactive or have some
>other effect that would cause degradation of cDNA over time, resulting
>in a smear if another gel were to be run? For that matter do the other
>common dyes have any longterm adverse effect on cDNA?
Just inhibits the Taq pol. Same as cresol red does if you have too much.
We have never found a blue dye that doesn't inhibit Taq. A lot of reds
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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