Another PCR Loading buffer question

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Wed Oct 28 05:09:58 EST 1998


In article <36365A7A.919D750D at sph.unc.edu>, John Scott Meschke
<jmeschke at sph.unc.edu> writes
>Second, it is clear from responses to my first posting that bromophenol
>blue is not compatible with PCR, but is it photoreactive or have some
>other effect that would cause degradation of cDNA over time, resulting
>in a smear if another gel were to be run?  For that matter do the other
>common dyes have any longterm adverse effect on cDNA?

Just inhibits the Taq pol. Same as cresol red does if you have too much.
We have never found a blue dye that doesn't inhibit Taq. A lot of reds
also inhibit. 

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk



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