Sequential Gene Disruption in Yeast

Peter Russell yeast at reed.edu
Wed Oct 28 11:52:28 EST 1998


I have a yeast strain that is leu2 trp1 cyh canR and I would like
to add other markers i.e. ura3, his3, lys2, ade2.  Can anyone 
recommend a sequential gene disruption procedure to use and, does anyone
have the appropriate disruption plasmids for what we want
to do?  [BTW: A multiply marked strain with all those markers will
not work for us because the strain has cytoplasmic linear plasmids.]

TIA

Peter Russell
yeast at reed.edu



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