Dear netters, I would like to know wich is the best protocol to detect mutation(s) at the mRNA level. I also would like to know wether there is a protocol for direct sequencing of RT-PCR product (without subcloning) wich might allow me to have the complete sequence of these products. T.I.A. PS: Please, answer me by E-mail D Harmegnies dharmegn at ben.vub.ac.be