Qiaex II

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Thu Oct 29 11:36:02 EST 1998


Michael <blaesse at biochem.mpg.de> wrote:
> From my experience it is not possible to quantify DNA using OD260 after 
> extracting the DNA out of gel using a kit with glas milk. Perhaps there 
> are some particles left in the supernatant.

You should be able to see this by measuring the OD at 320 nm (where DNA
does not absorb at all). (IMO this is one of the reasons why doing a
UV spectrum is almost always better than simply measuring OD260 or
even OD260 and OD280.)

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */



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