Qiaex II

Michael blaesse at biochem.mpg.de
Thu Oct 29 08:08:16 EST 1998


Hi all

> In article <36373718.0 at pfaff.ethz.ch>, beat.reidy at ipw.agrl.ethz.ch (Beat )
> wrote:
> 
> : Hi all (with greetings to Mechthild),
> : 
> : is it true that quantification of DNA using OD 260 values after Qiaex II gel 
> : extraction do generally over-estimate the yield? Using gel quantification I 
> : ended up in about half of the concentration... 
> : 
> 
> IIRC, DNA from QII comes off in H2O.  260 values can be difficult.  One
> should always do the dilution in TE vs H2O, and aim for an A260 reading in
> the linear range of the instrument (generally 0.1 < A260 1.0).  It helps
> to check the calibration of the spec. against known standards, too.
> 
> Oh, and check your controls for the gel . . . :-)
> 



More information about the Methods mailing list