blaesse at biochem.mpg.de
Thu Oct 29 08:08:16 EST 1998
> In article <36373718.0 at pfaff.ethz.ch>, beat.reidy at ipw.agrl.ethz.ch (Beat )
> : Hi all (with greetings to Mechthild),
> : is it true that quantification of DNA using OD 260 values after Qiaex II gel
> : extraction do generally over-estimate the yield? Using gel quantification I
> : ended up in about half of the concentration...
> IIRC, DNA from QII comes off in H2O. 260 values can be difficult. One
> should always do the dilution in TE vs H2O, and aim for an A260 reading in
> the linear range of the instrument (generally 0.1 < A260 1.0). It helps
> to check the calibration of the spec. against known standards, too.
> Oh, and check your controls for the gel . . . :-)
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