Sequential Gene Disruption in Yeast

Anja Lorberg lorberga at uni-duesseldorf.de
Thu Oct 29 03:57:45 EST 1998


Peter Russell wrote:

> I have a yeast strain that is leu2 trp1 cyh canR and I would like
> to add other markers i.e. ura3, his3, lys2, ade2.  Can anyone
> recommend a sequential gene disruption procedure to use and, does
> anyone
> have the appropriate disruption plasmids for what we want
> to do?  [BTW: A multiply marked strain with all those markers will
> not work for us because the strain has cytoplasmic linear plasmids.]
>
> TIA
>
> Peter Russell
> yeast at reed.edu

   Dear Peter,

we always use a method according to Güldener et al., Nucleic Acids
Research 1996, Vol..24, No.13: 2519-2524.
                        Good luck,            Anja




More information about the Methods mailing list