protein purification

Balazs Siminszky balazs_siminszky at ncsu.edu
Thu Oct 29 16:04:30 EST 1998


Hi,

I am unsuccessful in purifying a His-tagged protein expressed in E.
coli.  I am using the INVITROGEN pRSET plasmid + resin system without
any product at the end.  I was using the Invitrogen protocol.  Positive
controls do work, however.  Any idea what to try changing?  Any system
better than the resin?

Thanks
B.



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