Purifying antibodies using blotted protein

R.N. Leach BMBRNL at leeds.ac.uk
Fri Oct 30 05:36:39 EST 1998


Is it possible to use protein blotted onto PVDF or nitrocellulose as a means 
of affinity purifying those antibodies that bind to it?
Is the choice of blotting membrane crucial?
Would the elution conditions be so harsh as to damage the antibodies?
Any information gratefully received.

Rob Leach,Ph.D.,
School of Biochemistry & Molecular Biology,
University of Leeds,
LEEDS, W.Yorks.,
England



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