PCR: SOE, Bridge or Megaprimer?

Cindy Boardman boardman.3 at osu.edu
Fri Oct 30 16:39:20 EST 1998


Request for help:

I am interested in expressing a chimeric protein and have been trying to
create the junction between the two DNAs by PCR.  I have tried Bridge, SOE
and Megapriming PCR and have had no luck.  Any generous and enlightened
souls out there willing to save me?

Thanks a lot.



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