Rich Dudley rdudley+ at pitt.edu
Tue Sep 1 08:49:35 EST 1998

I have used GST, 6xHis, affinity, and plain old ion-exchange.  Why?  Each
protein is different.  It doesn't matter what one person used best for a
particular protein--it may not work for yours.  You really need to look at
people who have purified your same, or similar proein, if possible, and you may
have to try a few things.  Protein work isn't as cut and dry as most DNA work


Michael Vork wrote:

> Hi there,
> I would like to have as many personal experiences as possible concerning the
> production and purification of recombinant eukaryotic protein in E. Coli.
> Which system is best and why (6xHis, GST, pET, IMPACT, other ??????)
> Thanks to you all in advance.

--- --- --- -- -- -- --- --- ---
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Dept. of Cell Biology and Physiology
University of Pittsburgh
---> search BIONET archives at http://www.bio.net <---

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