sadowski at REMOVEunixg.ubc.ca
Thu Sep 3 18:15:15 EST 1998
I recently purchased an ABI 310 sequencer for my lab. I like it a lot,
great seqeunces, simple to use, excellent quality sequencing with the Big
However, what I do not like about the instrument is that you have to use a
polymer called POP-6, a sample buffer callet TSR (template suppression
reagent), and an electrophoresis buffer called "310 Genetic Analyser
Buffer with EDTA". The latter is probably just TBE. I really would like
to know what all of these are, because if there is a possibility of
preparing them in the lab it would save a lot of expense and down time
when someone forgets to order something in time.
Anybody have any knowledge of the composition of these reagents? Any
other advice/ tips on use of the 310 would be welcome as well.
remove the REMOVE from my email address to respond.
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