Rapid Westerns on PVDF

Ian McFarlane i.mcfarlane at icrf.icnet.uk
Thu Sep 3 08:40:09 EST 1998


Hi,

I have a few questions about using PVDF (Immobilon P) without blocking (or
wetting) the membrane.

1) Compared to the wet blotting method do you need relativley higher or
lower concentrations of your primary and secondary Abs?

2) Does anyone have any experiences with ECL detection that they can
share? Specifically how much of the ECL reagent stays with the blot when
you wrap it for exposure and if, as seems likely, only a small amount does
stay on the blot how does that affect the length of time that you can get
a signal from the blot.

Thanks,

Ian Mc



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