Protein Electrophoresis

noone noone at cancer.bham.ac.uk
Fri Sep 4 07:34:59 EST 1998


In article <bpmurray*STUFFER*-0309980305010001 at macmac-2.ucsf.edu>,
bpmurray*STUFFER*@socrates.ucsf.edu (Bernard P. Murray, PhD) wrote:

> In article <Pine.GSO.3.96.980903123035.2543B-100000 at sun3.oulu.fi>, Pete
> <pkursula at cc.oulu.fi> wrote:
> 
> > On Wed, 2 Sep 1998, marc abrams wrote:
> > How about a native gel without SDS? Just an idea...
> 
> Hey, if we're changing the gel system completely then how
> about switching to isoelectric focusing?  This sounds like
> the ideal situation.  However, I am not sure how easy it is
> transfer proteins from an IEF gel.
>      Bernard
> 

I think SERVA (now part of Novex or Boehringer Ingelheim) sells IEF foils
you can blot afterwards.

Peter



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