Methylene Blue for DNA gels
rdudley+ at pitt.edu
Fri Sep 4 09:32:24 EST 1998
I've never heard of using methylene blue, but I have heard of using crystal
violet. It's not that great--you need a ton of DNA to see a band. I found
a protocol at this website:
William A. Rosche, Ph.D. wrote:
> Dear Netters,
> I have been told that methylene blue works great for visualizing DNA
> when you are going to cut out a band.
> What concentration of methylene blue is used?
> Any other tips on the use of methylene blue.
> Thank you,
> William A. Rosche
--- --- --- -- -- -- --- --- ---
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Dept. of Cell Biology and Physiology
University of Pittsburgh
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