Methylene Blue for DNA gels

Rich Dudley rdudley+ at pitt.edu
Fri Sep 4 09:32:24 EST 1998


I've never heard of using methylene blue, but I have heard of using crystal
violet.  It's not that great--you need a ton of DNA to see a band.  I found
a protocol at this website:

    http://tto.trends.com/

rich

William A. Rosche, Ph.D. wrote:

> Dear Netters,
>
>     I have been told that methylene blue works great for visualizing DNA
> when you are going to cut out a band.
>
>     What concentration of methylene blue is used?
>
>     Any other tips on the use of methylene blue.
>
>     Thank you,
>
>         William A. Rosche



--
--- --- --- -- -- -- --- --- ---
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Dept. of Cell Biology and Physiology
University of Pittsburgh
http://www.cbp.pitt.edu
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