Help: ISH on frozen section

[jessehcl]

Dear News Reader,

I am now doing ISH on frozen sections of mouse aorta. The mouse aorta
was cut into 6 micro thick sections, mount on APES-coated slides, and
leave dry at room temperature for 30 min before post-fixation.
However,less than 50% sections left after intesive wash (before and
after hybridization).  Any advice is welcome.

Thank you very much for your assistance in advance.


Chih-L Han 
return mail to jessehcl at doctormail.com