Help! A question about SSCP

bianhk bianhk at 163.NET
Mon Sep 7 00:21:15 EST 1998


I am new to this newsgroup, please forgive me if my behavir is wrong or
rude.
I am looking for a mutation site of  BRCA1 and BRCA2 gene, the material
of DNA is mostly coming from paraffin.
I have done SSCP for hundreds of times ,some of them indicate the
mutation definitaly. However, the results of DNA sequence always beat
me:there is no mutation at all.
I know there must be someone who would tell me why.
Any suggestion will be highly appreciated.

Ma yihong,PhD student
DEP. of pathiology ,Beijing Medical University.




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