Biotin-Streptavidin-ECL:Problems,Help appreciated

Hendrik van den Berg h.van at qub.ac.uk
Mon Sep 7 06:27:44 EST 1998


Hi all

We are using a biotinylated peptide to probe for cellular binding
sites using streptaviidin-ECL detection. Both in fluorocytochemistry
and dot blots/westerns of detergent extracts we see a lot of
endogenous biotin expression in most of our breast cancer cell lines,
ie flourescence detected using streptavidin -ECL in the absence of the
probe. 5-6 bands on western, 20-90 kDa.

Anybody else had this problem? Could we block the non-specifics by
blocking with streptavidin? 

Help appreciated

Hendrik



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