limitation of subcloning into vector DNA(pBleuescript etc)

f1221 at CC.NAGASAKI-U.AC.JP f1221 at CC.NAGASAKI-U.AC.JP
Tue Sep 8 06:25:13 EST 1998


At 3:12 AM 98.9.8, Jun-Ichi Aikawa wrote:
> Hello,
>
> I have obtained BAC DNA and will try to subclone its partial DNA fragments
> into commercially available plasmid DNA (pBleuescript, pUC, pGEM etc). How
> much bp DNA fragment can I introduce into vector DNA? Does anyone tried
> more than 10kb genomic DNA?
>
> Thanks.
>
>
> Jun-ichi Aikawa, Ph.D.
> University of California, San Diego

Hello,
I have done the same thing recently and fragments of 10 to 13 kB were OK in
pUC19.

Best Regards, Jota







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