limitation of subcloning into vector DNA(pBleuescript etc)

[Jun-Ichi Aikawa]

Hello,

I have obtained BAC DNA and will try to subclone its partial DNA fragments
into commercially available plasmid DNA (pBleuescript, pUC, pGEM etc). How
much bp DNA fragment can I introduce into vector DNA? Does anyone tried
more than 10kb genomic DNA?

Thanks.


Jun-ichi Aikawa, Ph.D.
University of California, San Diego