limitation of subcloning into vector DNA(pBleuescript etc)

Jun-Ichi Aikawa jaikawa at UCSD.EDU
Tue Sep 8 05:12:42 EST 1998


Hello,

I have obtained BAC DNA and will try to subclone its partial DNA fragments
into commercially available plasmid DNA (pBleuescript, pUC, pGEM etc). How
much bp DNA fragment can I introduce into vector DNA? Does anyone tried
more than 10kb genomic DNA?

Thanks.


Jun-ichi Aikawa, Ph.D.
University of California, San Diego





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