Horrendous background problem on chemiluminescent westerns
colin at nospam.fungus.usask.ca
Wed Sep 9 12:05:38 EST 1998
In article <35f570a7.2937673 at news.iastate.edu>, ckafer at iastate.edu (Guess)
> Hi. I am hoping somebody can give some tips on cutting down the
> background on my chemiluminescent westerns (ECL reagent). I get large
> spots, patches and smears all over (as opposed to a nonspecific
> recognition/banding pattern). Its particularly bad where the edges
> of the gel contact the membrane during transfer.
How much of the primary and ECL antobodies are you using ?? We've found
that you get a better results with much less of the secondary antisera
than Amersham suggests.
University of Saskatchewan
More information about the Methods