About PCR from a single yeast colony

Mohammad H Ghahremani mghahrem at uottawa.ca
Wed Sep 9 13:38:11 EST 1998


In article <1C65015A0F31 at serverdos.cigb.edu.cu>, verena.muzio at cigb.edu.cu wrote:

> Dear colleagues:
> Could somebody send me a simple protocol for PCR from a single yeast
> colony. 
> Please, write to my e-mail address 
> Thanking in advance,
> Alexis

Hi Alexis

I do yeast colony PCR very often. I use 0.1% Triton-X in my PCR mix with no
enzyme (taq) and set a 10min 92°C in the start of the PCR program to break
the yeat cell membrane. When the 10min is about to finish I add the enzyme.
This way U get more of the enzyme.

Good luck,

Mohammad
      _ _
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