Hi all: I'm about to generate my own TA-vector for PCR product cloning. I'll digest pXcmkn12 with XcmI. But then I do not know whether I have to dephosporylate in order to prevent religation. Has anybody of you done that before ? Did you use Boeringer's shrimp alkaline phosphatase (easier to deactivate later) or any ordinary CIP ? Cheers, Markus