mouham at biomail.ucsd.edu
Thu Sep 10 18:35:04 EST 1998
In article <6spojr$ptg$1 at news.doit.wisc.edu>,
klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu (Dima Klenchin) wrote:
> In article <35EFFB31.532 at biotec.jouy.inra.fr>, Ina Hinners
<hinners at biotec.jouy.inra.fr> wrote:
> :Martinec at ueb.cas.cz wrote:
> :> Hi everybody,
> :> I need to remove easily ATP from solution to stop calcium loading by Ca2+-
> :> ATPases. Does anyone have an idea?
> :> Jan
> :Dear Jan,
> :I know that one can stop ATP-dependent reactions by adding glucose and
> :Hexokinase, but I dont remember the concentrations, but this has been
> :published quiet often. So take a look at this. Hope this helped you.
> :Rgegards, Ina
> 2-5 mM glucose and some hexokinase (I can look up my journals if needed).
> Also apyrase without glucose but it is less specific and will chew up some
> GTP as well.
> - Dima
Adding a non-hydrolyzable form of ATP (S-gamma-ATP for example) might
also do the trick.
More information about the Methods