n-myc PCR not working

nospam at nospam.net nospam at nospam.net
Thu Sep 10 12:32:47 EST 1998


Taking a stab in the dark.  I know this sounds like a moronic question.  I'm
trying to PCR full length human n-myc, and can't get any product while using
a primer to the 3' end of the thing at the stop codon.  The primer looks
fine.  Has the same Tm as the 5' primers I've tried.  Is there some reason
for why this sequence will not PCR?  Thanks.



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