Proteases in liver cytosols

Mike Green mgreen at animal.ufl.edu
Thu Sep 10 13:08:11 EST 1998


Hello Everyone,

	A simple question regarding the preparation of liver cytosols?  I use
10 mM phosphate, 150 mM NaCl to prepare uterine cytosols with no problem
of degradation.  Will this same buffer work for liver tissue or are
protease inhibitors needed?  Thanks for your input.

M.L. Green



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