Any problems with Pfu Turbo

January Weiner nospam_jweiner1 at ix.urz.uni-heidelberg.de
Thu Sep 10 05:49:27 EST 1998


We switched to Pfu - Turbo from Stratagene and seem to have many problems
with it. Under the specified conditions (te=1min/kbp, template 0,5 ug
genomic bacterial DNA) sometimes there is no product at all, even though it
ought to be quite small (~3.5 kb). The only non-standard thing is that one
of the oligonucleotides has a coupple of nucleotides more bearing a
restriction site.
	What are your experiences with Pfu Turbo?
	Thank you,
			January

-- 
----)-\//-///-----------------------------------January-Weiner-3-------
A machine is only a machine because it cannot think - G.K.Chesterton



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