Any problems with Pfu Turbo

Cres Bookstein cbookste at MEDICINE.BSD.UCHICAGO.EDU
Fri Sep 11 15:58:04 EST 1998


I have been using Pfu Turbo exclusively for the past few months on genomic
and RT targets, product sizes from 89bp to 5.3kb. I love it. All have been
sequenced, with no mistakes.  You may have to play with annealing
temperature to get the perfect product quantity.  The only problems I have
had were corrected with new set of primers, not with Taq, AmpliTaq Gold or
Vent.

Crescence Bookstein, Ph.D.
Research Associate/Assistant Professor
Dept of Medicine-GI  mc 6084
Univ. of Chicago
5841 S. Maryland Ave.
Chicago, IL 60637
cbookste at medicine.bsd.uchicago.edu

Phone: 773/702-2283             Fax: 773/702-2281





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