How packed Sepharose CL-4B

Sat Sep 12 13:22:16 EST 1998

Hello netters:
I am new, just began to worck at 24 august, my bust told me to pack a 
column of sepharose CL-4B of 100 cmX 16 mm of diameter of gel, and 
the sample will be on 2M Urea, 0,5 % of Triton X-1OO, 10 mM beta 
mercaptoetanol, 50 mM Tris-HCl, pH 8, 10 mM EDTA.
Please, how to get:
a) a good packed column with a number of plateu over 
3000?, b) good resolution for a protein near 1 MDa?.
Thanks in advance, Lazaro

Center for Genetic Engineering and Biotechnology.
Hepatitis C Department.
Vaccines Division.
PO Box 6162, ZP 10600
Cubanacan, Ciudad Habana, Cuba.
Fax: (53-7)218070 Phone: 53-7-218008/218446/218164
E-mail: Juan.Morales at

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