transfection of primary human fibroblasts

luvk at luvk at
Sat Sep 12 14:34:10 EST 1998

Is anyone aware of a good transfection technique for efficient transfection of
linear or circular plasmid DNA into primary human fibroblasts?  I have tried
Qiagen's Superfect and Effectene reagents, but the transfection efficiency for
my WI-38 and MRC-5 primary lung fibroblasts is not very good.  The reagents
work well for immortalized fibroblasts, however.

Thanks a lot for any info.

Manu Kohli

-----== Posted via Deja News, The Leader in Internet Discussion ==-----   Create Your Own Free Member Forum

More information about the Methods mailing list