transfection of primary human fibroblasts

luvk at my-dejanews.com luvk at my-dejanews.com
Sat Sep 12 14:34:10 EST 1998


Is anyone aware of a good transfection technique for efficient transfection of
linear or circular plasmid DNA into primary human fibroblasts?  I have tried
Qiagen's Superfect and Effectene reagents, but the transfection efficiency for
my WI-38 and MRC-5 primary lung fibroblasts is not very good.  The reagents
work well for immortalized fibroblasts, however.

Thanks a lot for any info.

Manu Kohli

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