Plasmid Isolation for over-expressed protein
gmanning at wnmeds.ac.nz
Sun Sep 13 16:13:14 EST 1998
Sean Yoder wrote in message <35FA0BC5.9BBB401A at chuma.usf.edu>...
> I'm having problems consistenltly isolating plasmid DNA from JM109
>host with a pCMV plasmid with a gene coding for a wall protein of S.
>mutans. The plasmid seems to be over-expressing the gene and
>consequently lysing cells prematurely possibly due to the thin cell wall
Try growing your bugs in isotonic concentrations of sucrose. This should
stop them popping prematurely.
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Wellington School of Medicine
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